Chloroform cometabolism by butane-grown bacteria

diversity in butane monooxygenases by Natsuko Hamamura

Written in English
Published: Pages: 81 Downloads: 930
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Subjects:

  • Chloroform -- Biodegradation.,
  • Bacteria -- Metabolism.,
  • Monooxygenases.

Edition Notes

Statementby Natsuko Hamamura.
The Physical Object
Pagination81 leaves, bound :
Number of Pages81
ID Numbers
Open LibraryOL15487618M

PFASs (perfluoroalkyl and polyfluoroalkyl substances) are highly fluorinated, aliphatic, synthetic compounds with high thermal and chemical stability as well as unique amphiphilic properties which make them ingredients in a range of industrial processes. PFASs have attracted consideration due to their persistence, toxicity and bioaccumulation tendency in the environment. A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients is developed and presented. The method was validated by applying it to data obtained from batch kinetics of the aerobic cometabolism of 1,1,1‐trichloroethane (1,1,1‐TCA) by a butane‐grown mixed culture. Butane-grown cells were provided 3 mM lactate and 13 μM 1,1-DCE or 10 μM 1,2-cis-DCE. The slopes of tangents drawn to progress curves obtained during the assays were measured at the indicated time points to determine rates of O 2 uptake. The results are expressed as percentages of the initial rate of respiration immediately following addition. Chloroform aerobic cometabolism by butane-growing Rhodococcus aetherovorans BCP1 in continuous-flow biofilm reactors. Bioprocess and Biosystems Engineering , 35 (5),

This study, focused on chloroform (CF) cometabolism by butane-grown bacteria, was conducted in a 2-m continuous-flow column reactor simulating a portion of saturated aquifer. The main goals were: a) to investigate the pulsed injection of growth substrate and oxygen as a tool to control clogging of the porous medium and to attain a wide. Butane-oxidizing Arthrobacter (ATCC ) bacteria were shown to degrade low concentrations of methyl t -butyl ether (MTBE; range, to μg/liter) with an apparent half-saturation concentration (K s) of mg/liter and a maximum substrate utilization rate (kc) of mg/mg of total suspended solids per day. Arthrobacter bacteria demonstrated MTBE degradation activity when grown on. Alkane-utilizing bacteria may degrade pollutants aerobically (or anaerobically), e.g., through direct metabolism, sequential metabolism, reductive metabolism, dehalogenation or cometabolism. Bacteria that may be suitable for use in the present septic system treatment process include the following Groups (in addition to fungi, algae, protozoa. Hamamura et al., Chloroform Cometabolism by Butane-Grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and Methane-Grown Methylosinus trichosporium OB3b, Applied and Environmental Microbiology, vol. 63, No. 9, pp. (Sep. ).

Chloroform cometabolism by butane-grown bacteria by Natsuko Hamamura Download PDF EPUB FB2

Chloroform (CF) degradation by a butane-grown enrichment culture, CF8, was compared to that by butane-grown Pseudomonas butanovora and Mycobacterium vaccae JOB5 and to that by a known CF degrader, Methylosinus trichosporium OB3b.

All three butane-grown bacteria were able to degrade CF at rates comparable to that of M. by: Hamamura N, Page C, Long T, Semprini L, Arp DJ.

Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB 5, and methane-grown Methylosinus trichosporium OB3b. Applied and Environmental Microbiology ;63(9) RC (Final) Full-text: AEM-Full Text PDF Exit; Abstract: AEM-Abstract Exit.

Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and methane-grown Methylosinus trichosporium OB3b. Appl. Environ. Microbiol. [PMC free article]Cited by: Aerobic Chloroform cometabolism by butane-grown bacteria book of chloroform by butane-grown microorganisms: long-term monitoring of depletion rates and isolation of a high-performing strain.

Frascari D(1), Zannoni A, Fedi S, Pii Y, Zannoni D, Pinelli D, Nocentini by: Butane monooxygenases of butane-grown Pseudomonas butanovora, Mycobacterium vaccae JOB5, and an environmental isolate, CF8, were compared at the physiological level.

The presence of butane monooxygenases in these bacteria was indicated by the following results. (i) O 2 was required for butane degradation. (ii) 1-Butanol was produced during butane by: Chloroform biodegradation by butane-grown bacteria 13 Microcosm study 13 Pseudomonas butanovora 15 Mycobacterium vaccae JOBS 15 2.

Chloroform cometabolism by butane-grown CFS, Pseudomonas butanovora, and Mycobacterium vaccae JOBS and methane-grown Methylosinus trichosporium OB3b 18 Abstract 19 Introduction A survey of aerobic cometabolism of chlorinated aliphatic hydrocarbons by a butane-grown mixed culture was performed.

The transformation of 1,1-dichloroethylene (1,1-DCE) and cis-1,2. Chloroform (CF) degradation by a butane-grown enrichment culture, CF8, was compared to that by butane-grown Pseudomonas butanovora and Mycobacterium vaccae JOB5 and to that by a known CF degrader, Methylosinus trichosporium OB3b.

All three butane-grown bacteria were able to degrade CF at rates comparable to that of M. trichosporium. CF degradation by all four bacteria required O(inf2). Chloroform (CF) degradation by a butane-grown enrichment culture, CF8, was compared to that by butane-grown Pseudomonas butanovora and Mycobacterium vaccae JOB5 and to that by a known CF degrader, Methylosinus trichosporium OB3b.

All three butane-grown bacteria were able to degrade CF at rates comparable to that of M. trichosporium. Abstract. This work focuses on chloroform (CF) cometabolism by a butane-grown aerobic pure culture (Rhodococcus aetherovorans BCP1) in continuous-flow biofilm goals were to obtain preliminary information on the feasibility of CF biodegradation by BCP1 in biofilm reactors and to evaluate the applicability of the pulsed injection of growth substrate and oxygen to biofilm reactors.

CF aerobic cometabolism is supported by growth on short-chain alkanes (i.e., methane, propane, butane, and hexane), aromatic hydrocarbons (i.e., toluene and phenol), and ammonia via the activity. Although aerobic biotransformation of CF is possible (e.g., cometabolism by a butane-grown strain), CF is more difficult to cometabolize than trichloroethene.

Biotransformation of CF by mixed or pure cultures under methanogenic (5, 21) and sulfate-reducing (20) conditions has been reported, however, only at low-mg/liter CF concentrations.

Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB 5, and methane-grown Methylosinus trichosporium OB3b. Applied and Environmental Microbiology ;63(9)   The ability of a Rhodococcusaetherovorans strain, BCP1, to grow on butane and to degrade chloroform in the 0– μM range (0– mg l−1) via aerobic cometabolism was investigated by means of resting-cell assays.

BCP1 degraded chloroform with a complete mineralization of the organic Cl. The resulting butane and chloroform maximum specific degradation rates were equal to. Butane monooxygenases of butane-grown Pseudomonas butanovora, Mycobacterium vaccae JOB5, and an environmental isolate, CF8, were compared at the physiological level.

The presence of butane monooxygenases in these bacteria was indicated by the following results. (i) O2 was required for butane degradation. (ii) 1-Butanol was produced during butane degradation. Chloroform Cometabolism by Butane-Grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and Methane-Grown Methylosinus trichosporium OB3b.

Appl Environ Microbiol. ; 63(9): Frascari D, Pinelli D, Nocentini M, Fedi S, Pii Y, Zannoni D. Chloroform degradation by butane-grown cells of Rhodococcus aetherovorans BCP1.

Hamamura N, Page C, Long T, Semprini L, Arp D () Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and methane-grown Methylosinus trichosporium OB3b. Appl Environ Microbiol – CAS Google Scholar.

Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB 5, and methane-grown Methylosinus trichosporium OB3b. Applied and Environmental Microbiology ;63(9)   In this paper, work on cometabolism is reviewed, and several strategies are presented that can be used to better exploit cometabolism.

The review focuses on aerobic bacteria, and more emphasis is given to important pollutants such as HMW-PAHs and chlorinated compounds. General concepts of cometabolism Biodegradation and cometabolism. Young Kim, Daniel J. Arp, Lewis Semprini, A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients for aerobic cometabolism of 1,1,1‐trichloroethane by a butane‐grown mixed culture, Biotechnology and Bioengineering, /bit, 77, 5.

Chloroform cometabolism by butane-grown bacteria: diversity in butane monooxygenases. A field study was performed to evaluate the potential for in-situ aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA) through bioaugmentation with a butane enrichment culture containing predominantly two Rhodococcus sp.

strains named BP and BP that could cometabolize 1,1,1-TCA and 1,1-dicholoroethene (1,1-DCE). Batch tests indicated that 1,1-DCE was more rapidly transformed.

A survey of aerobic cometabolism of chlorinated aliphatic hydrocarbons by a butane-grown mixed culture was performed. The transformation of 1,1-dichloroethylene (1,1-DCE) and cis-1,2-dichloroethylene (c-DCE) required O 2 and was inhibited by butane and inactivated by acetylene, indicating that a monooxygenase enzyme was likely involved in the transformations.

As illustrated by Frascari et al. in previous studies of CF cometabolic biodegradation by butane-grown bacteria in batch reactors, a high T c corresponds to a low primary substrate flow rate required to sustain the cometabolism of a given CAH flow rate, whereas a low b corresponds to a high steady-state biomass concentration obtained with a.

v ol. 63, chloroform cometabolism by butane-grown bacteria pected if both CF and the growth substrate compete to bind to the same enzyme (the monooxygenase). Measurement of the concentrations of salicylate, NO 3 − and NO 2 − during the cometabolism furnished the molar ratio of the joint degradation of NO 3 − and salicylate under anaerobic conditions.

In the optimization of the NO 3 −-N:salicylate ratios, the measured (SH, C23O) enzyme activities were also determined, since it was not necessary to take into account the catechol.

Progress Report: Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms EPA Grant Number: RC Subproject: this is subproject numberestablished and managed by the Center Director under grant R (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

The ability of a Rhodococcus aetherovorans strain, BCP1, to grow on butane and to degrade chloroform in the 0– μM range (0– mg l−1) via aerobic cometabolism was investigated by means. KIM et al. () showed that butane and propane were effective cometabolic substrates to drive the transformation of chloroform.

Recently, HAMAMURA et al. () have shown that butane-oxidizing bacteria can degrade chlori- nated aliphatic hydrocarbons. Butane growing bacteria may have potential in the bioremediation of these com- pounds.

Aerobic cometabolism of chloroform by butane-grown microorganisms: Long-term monitoring of depletion rates and isolation of a high-performing strain April Biodegradation 16(2).

Young Kim, Daniel J. Arp, Lewis Semprini, A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients for aerobic cometabolism of 1,1,1‐trichloroethane by a butane‐grown mixed culture, Biotechnology and Bioengineering, /bit, 77, 5.

Dario Frascari, Arianna Zannoni, Stefano Fedi, Youry Pii, Davide Zannoni, Davide Pinelli, Massimo Nocentini, Aerobic cometabolism of chloroform by butane-grown microorganisms: long-term monitoring of depletion rates and isolation of a high-performing strain, Biodegradation, /s, 16, 2, (), ().Acetylene was shown to work as a mechanism-based inactivator for all three strains of butane-grown bacteria, however the [14 C]acetylene labeling patterns were different among the three.

Chloroform cometabolism by butane-grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and methane-grown Methylosinum trichosporium OB3b.